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norskBlar i forfatter "Rothweiler, Ulli"
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2.09 Å resolution structure of E. coli HigBA toxin-antitoxin complex reveals an ordered DNA-binding domain and intrinsic dynamics in antitoxin
(Journal article; Tidsskriftartikkel; Peer reviewed, 2020-10-29)The toxin–antitoxin (TA) systems are small operon systems that are involved in important physiological processes in bacteria such as stress response and persister cell formation. Escherichia coli HigBA complex belongs to the type II TA systems and consists of a protein toxin called HigB and a protein antitoxin called HigA. The toxin HigB is a ribosome-dependent endoribonuclease that cleaves the ... -
The crystal structure of haemoglobin from Atlantic cod
(Journal article; Tidsskriftartikkel; Peer reviewed, 2019-07-16)The crystal structure of haemoglobin from Atlantic cod has been solved to 2.54 A˚ resolution. The structure consists of two tetramers in the crystallographic asymmetric unit. The structure of haemoglobin obtained from one individual cod suggests polymorphism in the tetrameric assembly. -
The crystal structure of the N-acetylglucosamine 2-epimerase from Nostoc sp. KVJ10 reveals the true dimer
(Journal article; Tidsskriftartikkel; Peer reviewed, 2019-01-08)<i>N</i>-Acetylglucosamine 2-epimerases (AGEs) catalyze the interconversion of <i>N</i>-acetylglucosamine and <i>N</i>-acetylmannosamine. They can be used to perform the first step in the synthesis of sialic acid from <i>N</i>-acetylglucosamine, which makes the need for efficient AGEs a priority. This study presents the structure of the AGE from <i>Nostoc</i> sp. KVJ10 collected in northern Norway, ... -
DYRK1A kinase inhibitors promote β-cell survival and insulin homeostasis
(Journal article; Tidsskriftartikkel; Peer reviewed, 2021-08-31)The rising prevalence of diabetes is threatening global health. It is known not only for the occurrence of severe complications but also for the SARS-Cov-2 pandemic, which shows that it exacerbates susceptibility to infections. Current therapies focus on artificially maintaining insulin homeostasis, and a durable cure has not yet been achieved. We demonstrate that our set of small molecule ... -
Enzyme-adenylate structure of a bacterial ATP-dependent DNA ligase with a minimized DNA-binding surface
(Journal article; Tidsskriftartikkel; Peer reviewed, 2014-11-01)DNA ligases are a structurally diverse class of enzymes which share a common catalytic core and seal breaks in the phosphodiester backbone of double-stranded DNA via an adenylated intermediate. Here, the structure and activity of a recombinantly produced ATP-dependent DNA ligase from the bacterium Psychromonas sp. strain SP041 is described. This minimal-type ligase, like its close homologues, ... -
Novel DYRK1A Inhibitor Rescues Learning and Memory Deficits in a Mouse Model of Down Syndrome
(Journal article; Tidsskriftartikkel; Peer reviewed, 2021-11-17)Down syndrome (DS) is a complex genetic disorder associated with substantial physical, cognitive, and behavioral challenges. Due to better treatment options for the physical co-morbidities of DS, the life expectancy of individuals with DS is beginning to approach that of the general population. However, the cognitive deficits seen in individuals with DS still cannot be addressed pharmacologically. ... -
Novel Scaffolds for Dual Specificity Tyrosine-Phosphorylation-Regulated Kinase (DYRK1A) Inhibitors
(Journal article; Tidsskriftartikkel; Peer reviewed, 2018-08-10)DYRK1A is one of five members of the dual-specificity tyrosine (Y) phosphorylation-regulated kinase (DYRK) family. The DYRK1A gene is located in the Down syndrome critical region and regulates cellular processes related to proliferation and differentiation of neuronal progenitor cells during early development. This has focused research on its role in neuronal degenerative diseases, including ... -
Structure and function of a CE4 deacetylase isolated from a marine environment
(Journal article; Tidsskriftartikkel; Peer reviewed, 2017-11-06)Chitin, a polymer of β(1–4)-linked N-acetylglucosamine found in e.g. arthropods, is a valuable resource that may be used to produce chitosan and chitooligosaccharides, two compounds with considerable industrial and biomedical potential. Deacetylating enzymes may be used to tailor the properties of chitin and its derived products. Here, we describe a novel CE4 enzyme originating from a marine ... -
The structure of a dual-specificity tyrosine phosphorylation-regulated kinase 1A-PKC412 complex reveals disulfide-bridge formation with the anomalous catalytic loop HRD(HCD) cysteine
(Journal article; Tidsskriftartikkel; Peer reviewed, 2015)Dual-specificity tyrosine phosphorylation-regulated kinase 1A (DYRK1A) is a protein kinase associated with neuronal development and brain physiology. The DYRK kinases are very unusual with respect to the sequence of the catalytic loop, in which the otherwise highly conserved arginine of the HRD motif is replaced by a cysteine. This replacement, along with the proximity of a potential disulfide-bridge ...
